Tender For Quotation Against Rate Contract For Exosomal Mirna Sequencing Scrvice--20Samples Niq For Equipmen ... in India

Work Detail

Tender For Quotation Against Rate Contract For Exosomal Mirna Sequencing Scrvice--20Samples Niq For Equipments/Consllmabi.Es/Spares/Accessories Items Costing From Rs.15, Oon.Oo To Rs.10. 00,000.0(1 Sample type - Exosomes. The requirements for exosomes like storage medium, should be mentioned properly in the quotation Protocol for total RNA extraction from the cells and kits used for the extraction process should be mentioned. The protocol for the isolation of miRNA from total RNA should also be mentioned in detail. cDNA Library preparation: library size ranging from 140 bp to 160 bp. The firm should have in-house Pippcn prep technology for accurate size selection for library preparation and the proof for the same should be attached with the quotation. Sequencing: The cDNA fragments should be sequenced by read length using sequence by synthesis method on a minimum of Hiseq 2500 Illumina or better sequencing facility with a minimum of IX 50 bp PE reads or more to obtain a minimum of 20- 30 million reads or more. Time frame: Raw data and Analysis should be delivered within 21 days post sample pick up. Quality control requirements: We expect a QC score of 35-40 for our data and want the RNA QC report. Library QC report, and Data QC report to be shared at every step. Proper documentation proof including prior experience regarding the same should be shared. Raw Data QC and Reporting. High quality clean reads Ulumina HiSeq with a 1x50 bp PE reads to obtain 20-30 million reads. Micro RNA Seq raw reads will be processed for quality check. miRDeep2 will be utilized for identification of novel and known micro RNAs from small RNA sequencing data in human. Venn Diagram showing all the micro-RNA unique and common to the various study group. Differential expression of microRNAs Significantly up and down regulated miRNA Validation of the differentially expressed miRNA amongst patients to control sample. Volcano plots and heat map for differentially expressed miRNA among the various groups Target identification and functional Annotation of the identified micro-RNA Pathway analysis Comprehensive compiled report and data Reference alignment metrics and various other data analysis representations required for the experiment and related literature will be reviewed by the client. i. . j i-i.

Contact Information