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E-Tender For Procurement Of Real Time-Pcr, Automated Tissue Extraction Machine And Other Accessories Under Animal Resources Development Deptt, Government Of Tripura , Automated Extraction System-- The Instrument Should Have An Open System Having Maximum Of 16 Magnetic Rods That Can Purify Up To 1-16 Samples Of Dna, Rna And Protein From Various Samples Such As Bacteria, Plant, Plasmid, Insect, Food, Saliva, Water, Blood, Serum, Plasma, Stool, Tissue, Buccal Swabs, Buffy Coat, Cells, Urine, Formalin- Fixed Paraffin- Embedded Tissue (Ffpe) And Other Body Fluids And The Purification Should Be Cross Contamination-Free. - The System Should Have A Cartridge Holder Adapter To Perform A Single Sample Without Wastageof Reagent & Plastic Ware. - The System Should Allow Movement Of Super Paramagnetic Particles Or Magnetic Beads Instead Of Transferring Liquids To Enable Efficient Removal Of Contaminants. - It Should Allow Purification And Processing Steps In 96-Well Deep Well Plates. - The System Should Accommodate Processing Volumes Of 50 To 1000?L - The System Should Have Heating For Both Lysis And Elution. -The Temperature Range For Both 96 Well Plates Should Be Ambient To 100°C. - The System Should Be Able To Process Up To 16 Samples At A Time. The Processing Time Should Be Less Than 45 Min. - The System Should Have Built-In Uv Disinfection Along With A Timer Setting. - The System Should Eliminate Hands-On Processes Such As Ethanol Precipitation, Phenol/Chloroform Extraction, Centrifugation Or Column Purification, Etc. - System Should Prevent Exposure To Hazardous Chemicals. - The Protocols Should Be Selectable From A Gui Touch Screen Display. - The System Should Be Portable And Easy To Move From One Place To Another Place. System Should Havece-Ivd Approval , Real Time Pcr System With Pcr Tubes-- System Should Have 96 Well Blocks With 0.2Ml Tubes, Strips And 96 Well Pcr Plate - The System Should Have Led Excitation With At Least 5 Led Filters For Excitation And 5 For Detection Filter, With 5 Color Multiplexing. The System Should Have - Ramping Rate Of The Machine Should Be 4°C/Sec With Temp. - Accuracy: ± 0.1°C; Temp. - Uniformity: ±0.3°C (Tested At 55°C); Temperature Range: 4-105°C With 0.1°C Min Increment - Ramping Rate : 4°C/Sec (Max) - Gradient Temp. Range 1-36°C. - Automatic 3D Hot Lid Technology To Reduce Evaporation. Hot Lid Temp. - Range 30-110°C (Adjustable Default 105°C), Automatic Hot Lid. - Sample Volume Should Be 5-100 ?L. - The Detection System Should Be Bottom-Based Photo Multiplier Tube Technology (Pmt). Where Individual Target Can Be Scanned. - Scanning Mode: Designated Line Scan Or Entire Plate. - Not Require Any Normalization Method. It Should Be Factory-Calibrated. No Passive Reference Should Be Needed. - Customization Of Dye Should Be Possible In The System. I.E., The Addition Of Dye To The Existing Library Should Be Possible - Dyes Used In System Should Be : F1: Fam, Sybr Green I;F2: Vic, Hex, Tet, Joe,Tamra;F3: Rox, Texas- Red;F4: Cy5, Quasar-670; F5: Cy5.5,Quasar-705. - Absolute Quantification Relative Quantification, Snp Analysis, Data Automatic Analysis, Multiple File Gene Expression Analysis, Melting Curve Genotyping, Hrm, Customized Parameters. - Softwareto Calculate ?Ct, ?Dct And Rq Fold Values After Completion Of Relative Quantification Assay For Automatic Post-Data Analysis And Bar Chart - Compatible With Reagents And Plastic Ware Of Any Make/Company. - Plus/Minus Assay. Snp (Should Be Capable Of Automatic And Manual Call With Simplified Scatter Plot). - Capability To Import Standard Curves In Multiple Experiments - Capability To Import And Export Experimental Data In Text Or Excel Format For Data Analysis - Provision With Software With No Download Restriction. - Software Capable Of Being Downloaded On Multiple Computers/Desktops/Laptops. - Presence Of Local Technical Application Support Within 24 Hours - The Provision Of Providing The Iqoqpq Documents At The Time Of Installation At Free Of Cost Along With Instrument Operation Training. , Single Channel Micropipette (0.1-2.5 ?L, 0.5-10 ?L, 10-100 ?L, 20 - 200 ?L 100-1000 ?L)-- Variable Volume Microlitre Pipettes Wherein Volumes Can Be Continuously Adjusted By Rotating The Plunger Or Thumb Button. It Has A Click-Stop Digital System, Reverse Pipetting Technique And Separate, Streamlined Tip Ejector. Each Pipette Has To Be Tested & Calibrated And Should Come With Its Own Calibration Certificate
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