Procurement Of Mammalian Gene Expressing Construct Procurement Of Mammalian Gene Expressing Construct , Mammalian Gene Expressing Construct -Subcloning And Codon Optimization Of Genes For Mammalian And Bacterial Expression, Subcloning Vector Pcdna 3.1;Pqe80lall The Sequences Should Be Codon-Optimized For Higher-Level Expression ( Soluble Form ) In E. Coli Using Gene Optimizer Technology Software And It Should Be Part Of The Synthetic Construct Optimization. Gene Number 2 To 9 Should Be Cloned In The Pqe80l Using 5’ Bamh1 / 3’Kpni. Gene Number 1 Should Be Cloned In The Pqe80l And Petsumo Or Pet151 Using 5’ Ecor1 / 3’Kpni. , Gene 1 Gaattc Attaaa Gag Gag Aaa Ttaact Atg Aga
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